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Table 1 Comparison of batch and fed-batch cultures of C. salexigens DSM 3043

From: Understanding the interplay of carbon and nitrogen supply for ectoines production and metabolic overflow in high density cultures of Chromohalobacter salexigens

 

Batch

Fed batch A

Fed batch B

Fed batch C

Set μ (h−1)

N/A

0.1

0.05

0.05

Initial [glucose] (mM)

20

20

20

20

Initial [ammonium] (mM)

30

30

30

15

Maximum biomass (g L−1)

2.12 ± 0. 2

12.6 ± 1. 6

28.1 ± 3.0

42.4 ± 2.0

Ectoine production (g L−1)

0.16 ± 0. 02

0.27 ± 0. 02

1.62 ± 0. 2

1.96 ± 0. 6

Hydroxyectoine production (g L−1)

0.18 ± 0. 02

0.18 ± 0. 03

1.51 ± 0. 3

2.25 ± 0. 7

Overall (gCDW g −1glucose )

0.59

0.39

0.50

0.76

Time (h)a

36

52

124

102

Stirring (rpm)

41–554

41–860

41–1138

41–1149

pO2 setting (%O2)

30

30

30

30

Aeration (L min−1)

1–6

1–4

1–2

1–3.5

  1. Experiments were performed in duplicate (batch and fed-batch A) or triplicate (fed-batch B and C). Data are average of experimental replicates
  2. aTime taken to reach maximum biomass