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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Enhanced extracellular production of recombinant proteins in Escherichia coli by co-expression with Bacillus cereus phospholipase C

Fig. 1

Expression of B. cereus PLC in E. coli BL21(DE3). a Kinetics of DCW and extracellular PLC activity. Error bars represent the standard deviation from three replicate measurements. b SDS-PAGE analysis of the culture supernatants of E. coli/pETDuet/plc (lane 1) and E. coli/pETDuet-1 (lane 2), as well as the culture supernatant (lane 3) and intracellular fraction (lane 4) of E. coli/pETDuet/plcD55L. c Inner membrane permeability of E. coli. d Outer membrane permeability of E. coli. e TEM-images of E. coli

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