Fig. 3From: Thermus thermophilus as source of thermozymes for biotechnological applications: homologous expression and biochemical characterization of an α-galactosidase a Determination of the TtGalA catalytic activity in the temperature range of 50 to 100 °C. pNP-α-d-galactopyranoside was used as substrate dissolved in 50 mM sodium phosphate buffer pH 6.0. Relative activity at 90 °C was considered as 100%. b The relative activity was measured between pH 3.0 and pH 9.0, at 90 °C considering as 100% the activity at pH 6.0Back to article page