Fig. 1From: A high-throughput expression screening platform to optimize the production of antimicrobial peptidesGolden Gate assembly of the expression plasmid in a single reaction. Six donor plasmids and one acceptor plasmid were combined to form each expression plasmid by directional cloning in a restriction-ligation step using the endonuclease BsaI and T4 DNA ligase. The overhangs produced by BsaI are coloredBack to article page