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Table 3 Media and growth conditions for yeasts and bacteria used in this study

From: Adipic acid tolerance screening for potential adipic acid production hosts

Microorganism Medium Growth conditions E-flask
1st pre-culture 2nd pre-culture Microplate Temperature (°C) Shaking (rpm)
E. coli LB medium (pH 7) Modified M9 medium (pH 7) Modified M9 medium (pH 6 or 7) 37 200
C. clutamicum Complex medium (pH 7) Minimal medium (pH 7) Minimal medium (pH 6 or 7) 30 200
Yeasts Minimal medium (pH 5.5) Minimal medium (pH 5) Minimal medium (pH 5 or 6) 30 180