Table 3 Media and growth conditions for yeasts and bacteria used in this study
From: Adipic acid tolerance screening for potential adipic acid production hosts
Microorganism | Medium | Growth conditions E-flask | |||
|---|---|---|---|---|---|
1st pre-culture | 2nd pre-culture | Microplate | Temperature (°C) | Shaking (rpm) | |
E. coli | LB medium (pH 7) | Modified M9 medium (pH 7) | Modified M9 medium (pH 6 or 7) | 37 | 200 |
C. clutamicum | Complex medium (pH 7) | Minimal medium (pH 7) | Minimal medium (pH 6 or 7) | 30 | 200 |
Yeasts | Minimal medium (pH 5.5) | Minimal medium (pH 5) | Minimal medium (pH 5 or 6) | 30 | 180 |