Table 4 Effect of different compounds on Chi18H8 activity

Control

100.0

Metal ions

mM

 Ca²⁺

20

126.8 ± 0.8

 Cu²⁺

20

0.1

 Fe²⁺

20

0.1

 K⁺

20

124.2 ± 3.1

 Mg²⁺

20

86.4 ± 1.4

 Mn²⁺

20

49.9 ± 0.1

 Ni²⁺

20

64.3 ± 0.1

 NH₄ ⁺

20

68.0 ± 7.7

 Zn²⁺

20

16.6 ± 2.0

 Co²⁺

20

119.6 ± 1.5

Chelating agents:

mM

 EDTA

20

53.6 ± 0.1

Reducing agents

mM

 2-mercaptoethanol

20

1.1 ± 2.1

DTT

10

20.5 ± 6.7

Detergents

% (w/v)

 SDS

1

0.0

 Triton X-100

1

72.2 ± 2.5

 Tween-20

1

24.0 ± 3.6

 DOC

1

120.4 ± 0.1

 Nonidet P-40

1

91.0 ± 2.0

 NLS

1

0.0

Sugars

mM

 GlcNAc

10

104.1 ± 3.3

 Chitobiose

10

82.0 ± 1.9

Organic solvents:

% (v/v)

 Ethanol

10

90.9 ± 2.5

 Methanol

10

88.6 ± 2.3

 n-Propanol

10

69.4 ± 1.9

DMSO

10

97.7 ± 14.4

Salt

M

 NaCl

0.1

38.5 ± 1.8

 NaCl

0.25

29.9 ± 8.1

 NaCl

0.5

16.6 ± 0.1

 NaCl

1

8.6 ± 0.4

 NaCl

2

4.4 ± 2.5