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Table 4 Effect of different compounds on Chi18H8 activity

From: Production and characterization of a novel antifungal chitinase identified by functional screening of a suppressive-soil metagenome

Compounds Final concentration Relative activity (%)
Control   100.0
Metal ions mM  
 Ca2+ 20 126.8 ± 0.8
 Cu2+ 20 0.1
 Fe2+ 20 0.1
 K+ 20 124.2 ± 3.1
 Mg2+ 20 86.4 ± 1.4
 Mn2+ 20 49.9 ± 0.1
 Ni2+ 20 64.3 ± 0.1
 NH4 + 20 68.0 ± 7.7
 Zn2+ 20 16.6 ± 2.0
 Co2+ 20 119.6 ± 1.5
Chelating agents: mM  
 EDTA 20 53.6 ± 0.1
Reducing agents mM  
 2-mercaptoethanol 20 1.1 ± 2.1
DTT 10 20.5 ± 6.7
Detergents % (w/v)  
 SDS 1 0.0
 Triton X-100 1 72.2 ± 2.5
 Tween-20 1 24.0 ± 3.6
 DOC 1 120.4 ± 0.1
 Nonidet P-40 1 91.0 ± 2.0
 NLS 1 0.0
Sugars mM  
 GlcNAc 10 104.1 ± 3.3
 Chitobiose 10 82.0 ± 1.9
Organic solvents: % (v/v)  
 Ethanol 10 90.9 ± 2.5
 Methanol 10 88.6 ± 2.3
 n-Propanol 10 69.4 ± 1.9
DMSO 10 97.7 ± 14.4
Salt M  
 NaCl 0.1 38.5 ± 1.8
 NaCl 0.25 29.9 ± 8.1
 NaCl 0.5 16.6 ± 0.1
 NaCl 1 8.6 ± 0.4
 NaCl 2 4.4 ± 2.5
  1. The activity was measured by the fluorimetric assay on 4-MU-(GlcNAc)2. Activity values are reported as relative to the value of 40.7 U/mg protein (set as 100%) recorded for purified Chi18H8 at 37 °C in 100 mM sodium acetate, pH 5.0. Values represent the means of three independent experiments (mean ± standard error)