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Table 3 Comparison of Chi18H8 production and IB solubilization in 2 L flasks, 3 L bench-bioreactor or 30 L industrial-bioreactor

From: Production and characterization of a novel antifungal chitinase identified by functional screening of a suppressive-soil metagenome

  Cell weight (gcell/L) Chi18H8 production (mg/gcell) Solubilization yield (%) Specific activity (U/mgprotein)
Flask 4.0 9.4 >80 40.7
3 L bench-bioreactor 5.3 9.1 >65 52.1
30 L industrial-bioreactor 6.2 12.9 >65 48.4
  1. Protein expression was induced in the early exponential growth phase (OD600nm ~0.6) with 0.4 mM IPTG and cells were harvested after 24 h of incubation. Chi18H8 production (mg protein/g cell) and solubilization yield in 10 mM lactic acid were estimated by densitometric analysis of proteins separated by SDS-PAGE. Specific activity of the solubilized enzyme was measured by the fluorimetric assay on 4-MU-(GlcNAc)2