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Table 3 Comparison of Chi18H8 production and IB solubilization in 2 L flasks, 3 L bench-bioreactor or 30 L industrial-bioreactor

From: Production and characterization of a novel antifungal chitinase identified by functional screening of a suppressive-soil metagenome

 

Cell weight (gcell/L)

Chi18H8 production (mg/gcell)

Solubilization yield (%)

Specific activity (U/mgprotein)

Flask

4.0

9.4

>80

40.7

3 L bench-bioreactor

5.3

9.1

>65

52.1

30 L industrial-bioreactor

6.2

12.9

>65

48.4

  1. Protein expression was induced in the early exponential growth phase (OD600nm ~0.6) with 0.4 mM IPTG and cells were harvested after 24 h of incubation. Chi18H8 production (mg protein/g cell) and solubilization yield in 10 mM lactic acid were estimated by densitometric analysis of proteins separated by SDS-PAGE. Specific activity of the solubilized enzyme was measured by the fluorimetric assay on 4-MU-(GlcNAc)2
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Microbial Cell Factories

ISSN: 1475-2859

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