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Table 2 Purification of Chi18H8 from IBs of E. coli BL21 Star™(DE3)/pET24b(+)::chi18H8 cells

From: Production and characterization of a novel antifungal chitinase identified by functional screening of a suppressive-soil metagenome

  Volume Activity Protein concentration Specific activity Purification Recovery
(mL) (U tot) (mg/mL) (U/mgprotein) (fold) (%) (mg/gcell)
IBs 2.0 4.7 11.2 0.2 1.0 100.0 9.4
Soluble fraction from IBs 10.0 314.0 1.1 28.5 66.8 82.0 7.7
Purified Chi18H8 25.3 355.7 0.2 63.9 75.7 57.4 5.4
  1. Enzymatic activity was measured by fluorimetric assay on 4-MU-(GlcNAc)2. Protein concentration was determined by the Biuret assay