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Table 2 Purification of Chi18H8 from IBs of E. coli BL21 Star™(DE3)/pET24b(+)::chi18H8 cells

From: Production and characterization of a novel antifungal chitinase identified by functional screening of a suppressive-soil metagenome

 

Volume

Activity

Protein concentration

Specific activity

Purification

Recovery

(mL)

(U tot)

(mg/mL)

(U/mgprotein)

(fold)

(%)

(mg/gcell)

IBs

2.0

4.7

11.2

0.2

1.0

100.0

9.4

Soluble fraction from IBs

10.0

314.0

1.1

28.5

66.8

82.0

7.7

Purified Chi18H8

25.3

355.7

0.2

63.9

75.7

57.4

5.4

  1. Enzymatic activity was measured by fluorimetric assay on 4-MU-(GlcNAc)2. Protein concentration was determined by the Biuret assay