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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Production and characterization of a novel antifungal chitinase identified by functional screening of a suppressive-soil metagenome

Fig. 4

Chi18H8 production at 3 L bench-bioreactor scale. a Chi18H8 production (mg/g cell) into insoluble fractions was determined by densitometric analysis of proteins separated through SDS-PAGE. Chitinolytic activity was measured by fluorimetric assay on 4-MU-(GlcNAc)2. b Time course of pH, pO2, temperature and wet weight. Induction of protein expression with 0.4 mM IPTG is indicated by an arrow. The values represent the mean of three experiments. Standard error never exceeded 10%

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