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Table 1 Strains used in this work

From: SWITCH: a dynamic CRISPR tool for genome engineering and metabolic pathway control for cell factory construction in Saccharomyces cerevisiae

Strains Genotype Source
PJ69-4 MATa trp1-901 leu2-3,112 ura3-52 his3-200 gal4D gal80D LYS2::GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ [28]
PJ69-4 S-1 MATa trp1-901 leu2-3,112 ura3-52 his3-200 gal4D gal80D LYS2::GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ X-3::pTEF1-hcas9-tCYC1 This study
PJ69-4 S-2 MATa trp1-901 leu2-3,112 ura3-52 his3-200 gal4D gal80D LYS2::GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ X-3::pTEF1-dcas9-VP64-tCYC1 This study
S-0 MATα Δura3 Δpad1 Δfdc1 Δleu2 Δaro10 This study
S-1 MATα Δura3 Δpad1 Δfdc1 Δleu2 Δaro10 X-3::pTEF1-hcas9_tCYC1 This study
S-2 MATα Δura3 Δpad1 Δfdc1 Δleu2 Δaro10 X-3::pTEF1-hcas9-tCYC1 XI-2::[pTDH3-AtPAL2-tPGI1 TEF2-C4H L5 ATR2-tCYC1 pPGK1-HaCHS-tENO2 pTEF1-PhCHI-tFBA1 pPDC1-At4Cl2-tTDH2] This study
S-3 MATα Δura3 Δpad1 Δfdc1 Δleu2 Δaro10 X-3::pTEF1-dcas9-tCYC1 XI-2::[pTDH3-AtPAL2-tPGI1 TEF2-C4H L5 ATR2-tCYC1 pPGK1-HaCHS-tENO2 pTEF1-PhCHI-tFBA1 pPDC1-At4Cl2-tTDH2] This study
S-4 MATα Δura3 Δpad1 Δfdc1 Δleu2 Δaro10 XI-2::[pTDH3-AtPAL2-tPGI1 TEF2-C4H L5 ATR2-tCYC1 pPGK1-HaCHS-tENO2 pTEF1-PhCHI-tFBA1 pPDC1-At4Cl2-tTDH2] This study