Table 1 Strains used in this work
Strains | Genotype | Source |
|---|---|---|
PJ69-4 | MATa trp1-901 leu2-3,112 ura3-52 his3-200 gal4D gal80D LYS2::GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ | [28] |
PJ69-4 S-1 | MATa trp1-901 leu2-3,112 ura3-52 his3-200 gal4D gal80D LYS2::GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ X-3::pTEF1-hcas9-tCYC1 | This study |
PJ69-4 S-2 | MATa trp1-901 leu2-3,112 ura3-52 his3-200 gal4D gal80D LYS2::GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ X-3::pTEF1-dcas9-VP64-tCYC1 | This study |
S-0 | MATα Δura3 Δpad1 Δfdc1 Δleu2 Δaro10 | This study |
S-1 | MATα Δura3 Δpad1 Δfdc1 Δleu2 Δaro10 X-3::pTEF1-hcas9_tCYC1 | This study |
S-2 | MATα Δura3 Δpad1 Δfdc1 Δleu2 Δaro10 X-3::pTEF1-hcas9-tCYC1 XI-2::[pTDH3-AtPAL2-tPGI1 TEF2-C4H L5 ATR2-tCYC1 pPGK1-HaCHS-tENO2 pTEF1-PhCHI-tFBA1 pPDC1-At4Cl2-tTDH2] | This study |
S-3 | MATα Δura3 Δpad1 Δfdc1 Δleu2 Δaro10 X-3::pTEF1-dcas9-tCYC1 XI-2::[pTDH3-AtPAL2-tPGI1 TEF2-C4H L5 ATR2-tCYC1 pPGK1-HaCHS-tENO2 pTEF1-PhCHI-tFBA1 pPDC1-At4Cl2-tTDH2] | This study |
S-4 | MATα Δura3 Δpad1 Δfdc1 Δleu2 Δaro10 XI-2::[pTDH3-AtPAL2-tPGI1 TEF2-C4H L5 ATR2-tCYC1 pPGK1-HaCHS-tENO2 pTEF1-PhCHI-tFBA1 pPDC1-At4Cl2-tTDH2] | This study |