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Fig. 6 | Microbial Cell Factories

Fig. 6

From: SWITCH: a dynamic CRISPR tool for genome engineering and metabolic pathway control for cell factory construction in Saccharomyces cerevisiae

Fig. 6

Downregulation of TSC13 by dCas9 interference. Transformants expressing interference gRNAs were cultured in fed-batch media and HPLC analysis was used to detect: a naringenin, b phloretic acid and c coumaric acid. d TSC13 transcript levels relative to ACT1 were measured by qRT-PCR. Stars indicate significantly different levels of either compounds concentration or TSC13 expression (p values <0.05) in strain pairs containing dCas9 or not, as indicated

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Microbial Cell Factories

ISSN: 1475-2859

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