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Fig. 2 | Microbial Cell Factories

Fig. 2

From: SWITCH: a dynamic CRISPR tool for genome engineering and metabolic pathway control for cell factory construction in Saccharomyces cerevisiae

Fig. 2

Efficient setup for SWITCH. a Screening for gRNAs that efficiently targets Cas9 to the cas9 gene, using TAPE, see text for details. b Confirmation of X-3 locus restoration by genomic PCR of 12 clones randomly selected amongst the transformants obtained in the presence of a X-3 rescue fragment. The presence of a 1.5 kb PCR fragment indicates that the X-3 locus has been restored

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Microbial Cell Factories

ISSN: 1475-2859

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