Joining the in vitro immunization of alpaca lymphocytes and phage display: rapid and cost effective pipeline for sdAb synthesis

Table 1 Sequences of primers used in the PCR

Primer	Sequence 5′–3′	Method
sDAb-Not-R	CCAGCGGCCGCTSWGGAGACRGTGACCWGGGTCC	Reverse transcription of RNA; VHH amplification
sDAb-NcoAsc-F	CGGCCATGGCCGGGCGCGCCGCCSAGGTGSAGSTSSWGSMGTC	VHH amplification
pSex Insertom F	ATGAAATACCTATTGCCTACGGCAG	Control PCR for electroporation
pSex Insertom R	CTACAACGCCTGTAGCATTCCAC	Control PCR for electroporation
UA Insertom F1	CGCATCACCATCACCATCACG	Control PCR for electroporation, plasmid pQE-30-mCherry-UA-GFP
UA Insertom R1	ACCAAATTGGGACAACACCAGTG	Control PCR for electroporation, plasmid pQE-30-mCherry-UA-GFP
sDAb-BamHI-F	AATGGATCCSAGGTGSAGSTSSWGSMGTC	VHH amplification for production of soluble sdAb
sDAb-SalI-R	GCTGTCGACCTATSWGGAAGACRGTGACCWGGGTCC	VHH amplification for production of soluble sdAb
H-OspA F	AGGATATCTAACGAAAAGGGTGAAACA	For production of H-OspA
H-OspA R without Stop	ATAGTCGACTTCTATGTCAGAGTCATCAAGTGC	For production of H-OspA with GFP fusion
H-OspA R with Stop	ATAGTCGACTCATTCTATGTCAGAGTCATCAAGTGC	For production of H-OspA without GFP fusion

ISSN: 1475-2859