Fig. 4From: Joining the in vitro immunization of alpaca lymphocytes and phage display: rapid and cost effective pipeline for sdAb synthesisPBMCs were cultured with (IL-2 and IL-4) or without interleukins (negative control) to assess any possible cytotoxicity of recombinant ILs as well as to confirm their ability to induce lymphocyte proliferation (d). Proliferation assay was performed using XTT. ILs were used with (gray columns) or without glycerol (black columns) to rule out any inhibitory effect of glycerol. * statistically significant induction (P < 0.01, paired t test) in the lymphocyte proliferation when compared to negative controlBack to article page