Fig. 2From: Joining the in vitro immunization of alpaca lymphocytes and phage display: rapid and cost effective pipeline for sdAb synthesisVector map of pSex81 plasmid (4864 bp). RBS, ribosome binding site; Signal peptide—pel-B leader sequence; Vh V and VI V regions, scFv single-chain fragment variable that serves as stuffer which is replaced by VHH sequence encoding sdAb; gene 3, gene for pIII surface protein. Note that sdAb are fused to pIII. T7 terminator; Bla gene, β-lactamase gene which gives ampicillin resistance; Col E1, Col E1 origin of replicationBack to article page