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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Joining the in vitro immunization of alpaca lymphocytes and phage display: rapid and cost effective pipeline for sdAb synthesis

Fig. 1

Vector map of pQE-30-mCherry-GFP plasmid (4880 bp). PT5 T5 promoter, lac O lac operator, RBS ribosome binding site, ATG Start codon, 6xHis His tag sequence, MCS I/MCS II multiple cloning sites; mCherry—red fluorescent protein that serve as stuffer, GFP, green flourescent protein; Stop codon; Col E1, Col E1 origin of replication; Ampicillin, ampicillin resistance gene. Note that incorporation of the stop codon at 3′ of insert produces recombinant protein without GFP fusion

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