Fig. 1From: Rhodococcus erythropolis as a host for expression, secretion and glycosylation of Mycobacterium tuberculosis proteinsAnalyses of purified rRhoApa from R. erythropolis transformed pNit-QC1-apa vector. a M. tuberculosis CS crude fraction and recombinant purified proteins blotted to PVDF membrane stained with Coomassie blue. Lane 1, M. tuberculosis H37Rv CS crude fraction. Line 2, purified protein from SE. Line 3, purified protein from IF. Line 4, purified protein from CS. Line 5, rE. coli Apa. b, c Lines are the same as a, but proteins were detected with Con A-HRP and with 6A3 mAb respectively. d Line 1, CS crude fraction from recombinant R. erythropolis and line 2, the flow through fraction from Ni-NTA column, both were detected with 6A3 mAb. Protein size markers are indicating on the left Back to article page