Fig. 5

Growth of DYB7 and DB1 producing hBD2 or rHA. rHA and hBD2 were expressed using 2 µm expression plasmids containing either the MET17 or PRB1 promoter upstream of the coding regions. These plasmids were transformed into S. cerevisiae strain DB1 or DYB7. The transformed yeast were inoculated at OD₆₀₀ = 0.15 into BMMD SFC with or without 1000 µM methionine and grown for 5 days, while OD₆₀₀ measurements were done approximately every 24 h. Error bars indicate standard deviations (n = 3). a Growth of DYB7 [pDB3936:GR:pDB4351], which express hBD2 from the MET17 promoter under non-repressing (0 µM methionine) and initially repressing conditions (1000 µM methionine). b Growth of DYB7 [pDB3936:GR:pDB4146], which express hBD2 from the PRB1 promoter without methionine (0 µM methionine) and with methionine in the media initially (1000 µM methionine). c Growth of DB1 [pDB3936:GR:pDB4351], which express hBD2 from the MET17 promoter under non-repressing (0 µM methionine) and initially repressing conditions (1000 µM methionine). d Growth of DYB7 [pDB3936:GR:pDB4692], which express rHA from the MET17 promoter under non-repressing (0 µM methionine) and initially repressing conditions (1000 µM methionine)