Fig. 6From: Engineered biosynthesis of milbemycins in the avermectin high-producing strain Streptomyces avermitilis Construction of S. avermitilis SAMA1M7ΔD. a Schematic diagram for construction of the aveD disruption mutant using pΔAveD. b PCR analysis for confirmation of the AveD inactivation using genomic DNA from S. avermitilis SAMA1M7ΔD. c DNA sequences of the aveD PCR product of S. avermitilis SAMA1M7. d DNA sequences of the inactivated aveD PCR product of S. avermitilis SAMA1M7ΔD. While TCA sequences exist in SAMA1M7 chromosome, the stop codon TGA sequences were generated in SAMA1M7ΔD chromosomeBack to article page