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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Engineered biosynthesis of milbemycins in the avermectin high-producing strain Streptomyces avermitilis

Fig. 6

Construction of S. avermitilis SAMA1M7ΔD. a Schematic diagram for construction of the aveD disruption mutant using pΔAveD. b PCR analysis for confirmation of the AveD inactivation using genomic DNA from S. avermitilis SAMA1M7ΔD. c DNA sequences of the aveD PCR product of S. avermitilis SAMA1M7. d DNA sequences of the inactivated aveD PCR product of S. avermitilis SAMA1M7ΔD. While TCA sequences exist in SAMA1M7 chromosome, the stop codon TGA sequences were generated in SAMA1M7ΔD chromosome

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