Fig. 4

Construction of S. avermitilis mutant strains. a Schematic diagram of aveA1 replacement using pSAMA1, and b PCR analysis for the confirmation of S. avermitilis SAMA1. c Schematic diagram of aveA3 replacement using pSAMA13, and d PCR analysis for the confirmation of S. avermitilis SAMA13. e Schematic diagram of aveA3-module7 replacement using pSAMA1M7, and f PCR analysis for the confirmation of S. avermitilis SAMA1M7