Table 2 Strains and plasmids used in this study
From: Recombinant production of the antibody fragment D1.3 scFv with different Bacillus strains
Strain or plasmid | Description | Reference/source |
|---|---|---|
Escherichia coli DH10B | F− endA1 deoR + recA1 galU galK16 nupG rpsL Δ(lac)X74 φ80lacZΔM15 araD139 Δ(ara, leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) StrR λ− | Invitrogen, Carlsbad, USA |
Bacillus megaterium MS941 | Bacillus megaterium DSM319 ΔnprM | [36] |
Bacillus megaterium ATCC14945 | Produces penicillin G acylase | American Type Culture Collection; Manassas, Virginia; USA |
Bacillus licheniformis MW3 | Bacillus licheniformis DSM13 (ΔhsdR1, ΔhsdR2) | [47] |
Bacillus subtilis 168 | trpC2 | [51] |
Bacillus subtilis DB431 | trpC2 nprE18 aprE epr bpr mpr ispA | [52] |
Bacillus subtilis WB800N | nprE aprE epr bpr mpr::ble nprB::bsr Δvpr wprA::hyg cm::neo; NeoR | |
pEJBmD1.3scFv | Shuttle vector for the recombinant production and secretion of D1.3 scFv in B. megaterium (TcR) | [33] |
p3STOP1623hp | Shuttle vector for cloning in E. coli (ApR) and production of target proteins in B. megaterium (TcR) controlled by the optimized xylose-inducible promoter; P opt xylA -mcs-stop; oriU/repU (B. cereus) | [32] |
pRBBm117 | p3STOP1623hp-derivative: shuttle vector for the recombinant production and secretion of D1.3 scFv in B. megaterium (TcR) controlled by the optimized xylose-inducible promoter; P opt xylA -sp lipA -d1.3scFv-his 6 -stop, oriU/repU (pBC16) | This work |
pALBm1 | p3STOP1623hp-derivative: shuttle vector for the recombinant production and secretion of penicillin G acylase in B. megaterium (TcR) controlled by the optimized xylose-inducible promoter; P opt xylA -sp pac -pac-stop, oriU/repU (pBC16) | This work |