Fig. 8

Time-dependent extracellular concentration of D1.3 scFv for the recombinant B. subtilis 168, DB431 and WB800N. Cultivation took place in microtiter plates (1250 µL culture volume), baffled shake flasks (150 mL of culture volume) and bioreactors (2 L culture volume) under aerobic conditions at 37 °C. All cultivation systems were inoculated with cell concentrations (cell dry weight) of 0.0337 g L⁻¹ in minimal medium containing 10 mg L⁻¹ of tetracycline. Recombinant D1.3 scFv secretion was induced by the addition of 5 g L⁻¹ of xylose at cell concentrations of around 0.07 g L⁻¹. Samples were taken at given time points to analyze extracellular concentration of D1.3 scFv [mg L⁻¹] for B. subtilis 168, DB431 and WB800N using ELISA