Fig. 5
From: Recombinant production of the antibody fragment D1.3 scFv with different Bacillus strains
Secretion of D1.3 scFv and other extracellular proteins by recombinant Bacillus strains. Extracellular proteins of 188 µL culture supernatant from shake flasks were precipitated and analyzed by SDS-PAGE. a Extracellular proteins of B. subtilis strains 168, DB431 and WB800N were analyzed by SDS-PAGE after 13–15 h of cultivation. Xylose-induced (Xyl+) and non-induced (Xyl−) cultures were compared with a protein standard (marker M). Only the proteins with a size between 25 and 32 kDa are shown. b Extracellular proteins of induced B. megaterium MS941 (22 h) and B. subtilis 168, DB431 and WB800N (13–15 h) were precipitated and analyzed by SDS-PAGE compared with a protein standard (marker M)