Fig. 4

Cultivation profile of recombinant B. licheniformis MW3 secreting D1.3 scFv. B. licheniformis MW3 was transformed with plasmid pRBBm117 (P ^opt. _xylA -sp _lipA -d1.3scFv-his ₆ ). Cultivation took place in microtiter plates (1250 μL culture volume), baffled shake flasks (150 mL of culture volume) and stirred tank bioreactors (2 L of culture volume) under aerobic conditions at 37 °C. All cultivation systems were inoculated with cell concentrations (cell dry weight) of 0.04 g L⁻¹ in minimal medium containing 10 mg L⁻¹ of tetracycline. Recombinant D1.3 scFv secretion was induced by the addition of 5 g L⁻¹ of xylose at cell concentrations of around 0.12 g L⁻¹. Samples were taken at given time points to analyze cell dry weight [g L⁻¹] and extracellular concentrations of D1.3 scFv [mg L⁻¹] using ELISA. Further analyses are shown in Additional file 1: Figure S2