Fig. 2

Cultivation profile of recombinant B. megaterium MS941 secreting D1.3 scFv in shake flasks. B. megaterium MS941 was transformed with plasmid pRBBm117 (P ^opt. _xylA -sp _lipA -d1.3scFv-his ₆ ). Cultivation took place in baffled shake flasks (150 mL culture volume) under aerobic conditions at 37 °C. Cultures were inoculated with cell concentrations (cell dry weight) of 0.0223 g L⁻¹ in minimal medium containing 10 mg L⁻¹ of tetracycline. Recombinant D1.3 scFv secretion was induced by the addition of 5 g L⁻¹ of xylose at cell concentrations of around 0.07 g L⁻¹. Samples were taken at given time points to analyze cell dry weight [g L⁻¹], extracellular concentrations of D1.3 scFv [mg L⁻¹] using ELISA and of fructose, xylose and acetate using HPLC