Fig. 1

Plasmid pRBBm117 for recombinant production of the antibody fragment D1.3 scFv. P ^opt. _xylA , optimized xylose-inducible promoter; opt. RBS, optimized ribosome binding site; sp _lipA , signal peptide sequence of B. megaterium extracellular esterase LipA; D1.3scFv, gene for single chain fragment variable (scFv) against lysozyme; his ₆ -tag, synthetic tag for 6× histidine residues; bla, β-lactamase gene for ampicillin resistance; ori (pBR322), E. coli origin of replication from plasmid pBR322; oriU (pBC16), B. megaterium origin of replication from plasmid pBC16; repU (pBC16), gene essential for plasmid replication in B. megaterium from plasmid pBC16; tetL, gene encoding tetracycline efflux pump for tetracycline resistance; xylR, gene encoding xylose repressor