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Fig. 4 | Microbial Cell Factories

Fig. 4

From: CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production

Fig. 4

Superpathway of methionine and SAM biosynthesis, with known transcriptional regulatory constraints indicated. Many genes involved in methionine biosynthesis and active, ATP-dependent uptake are part of the MetJ regulon and are thus subject to strong negative regulation (repression) by the eponymous ligand-responsive transcription factor MetJ (red) in presence of its cognate ligand SAM. Furthermore, the endogenous transcriptional activator of methionine synthase known as MetR (green) is repressed by MetJ:SAM, further attenuating methionine and SAM accumulation through de-activation of a critical node in the methionine biosynthetic pathway

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