Fig. 3
From: CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production
Gene dosage affects production of P3G. a Specifically, the upstream module was transferred from a low-copy vector into a single cassette with VvAOMT1 or CkmOMT2 on a high-copy vector, b increased copies of the upstream module led to ~fivefold improvement in P3G titer over the original constructs (Fig. 2). An additional, modest twofold improvement in overall P3G production (left), accompanied by a slight decrease in C3G to P3G conversion (right), was further achieved with VvAOMT1 (left) by utilizing an upstream module composed of MBP-fusions