Table 2 Primers used in this study
Primer name | Sequence (5′–3′) |
|---|---|
HAA1_del_fw | ACAGCACCAGCACTTGATTG |
HAA1_del_rv | ATGGTCTTACTCTCTGATACC |
HAA1_del_control_fw | ACAGAGTCGTGCATTTCCAC |
HAA1_del_control_rv | CCATGAGTGACGACTGAATC |
HAA1_BamHI_fw | CAGTGGATCCCTCTATGAGAAGAACCCACG |
HAA1_BamHI_rv | CAGTGGATCCTACACAACAAACTACGCAAGG |
HAA1_epPCR_fw | AAAAAGGAAACAAAAGTATAGAAAAAAAAAACCTAAAAAATAATG |
HAA1_epPCR_rv | AAACTACAGTTACAGAGAAGCAAGAGACGAAAAGCAAATTTATCA |
HAA1_C404T_GIN11_fw | CTGGCACAGAAAGCCAAAGAAGAAGCAAGAGCTAAAGCCAATCGGAACCCTAAAGGGAGC |
HAA1_G1447T_GIN11_fw | TTTACAGATTCATCGTCGATTTCAACGCTTTCCCGTGCAAATCGGAACCCTAAAGGGAGC |
HAA1_GIN11_rv | GGTACCAGGAAATGAAAGCG |
HAA1_kanMX_fw | GGGCTGCAGGAATTCGATATCAAGCTTATCGATACCGTCGCAGCTGAAGCTTCGTACGC |
HAA1_C404T_kanMX_rv | TCAGCTTTCGCTGGCAAGCTTACCGAACTATCTTGCCAGTGCATAGGCCACTAGTGGATCTG |
HAA1_G1447T_kanMX_rv | CAGCTAGGTTTGAAGGGTCCATCATCATATTTGCTATCGAGCATAGGCCACTAGTGGATCTG |
HAA1_control_fw | ACAGCACCAGCACTTGATTG |
GIN11_rv | CTGAAACGCAGATGTGCCTC |
HAA1_C404T_fw | TGTGAGAGGTGCATAAGAGG |
HAA1_C404T_rv | CCAATCGTAGACCAAAGAGC |
HAA1_G1447T_fw | GCTCTTTGGTCTACGATTGG |
HAA1_G1447T_rv | TGGTTCATCTCGTCTGGTAC |
HAA1_OE_GIN11_fw | AGCTTCTTTCCACGAAAGAAATAGTGTAAGTTAGAGGTACATCGGAACCCTAAAGGGAGC |
HAA1_OE_kanMX_rv | ATTAAGAAGCAAATACCTTCCTGTTGCTTGATTTGCCCTGGCATAGGCCACTAGTGGATCTG |
HAA1_OE_fw | ATGTAGTTCAACTTCTATGAATGCTCGGCGATACGATATGCTCTATGAGAAGAACCCACG |
HAA1_OE_rv | AAGGCTCATTTCCATGATGGGGTCACAATTATTATCGCACTACACAACAAACTACGCAAGG |
HAA1_OE_control_fw | GCAATCTCGACGATCAACTG |
HAA1_OE_control_rv | AAGAACCAGAATGGCAGGAC |
HAA1_GFP-NAT_fw | CGATCAAGGATTTGCGGATTTGGATAATTTCATGTCTTCGTTA CGG ATC CCC GGG TTA ATT AA |
HAA1_GFP-NAT_rv | CTACAGTTACAGAGAAGCAAGAGACGAAAAGCAAATTTA TCAGAATTCGAGCTCGTTTAAAC |
HAA1_STOP483_GFP-NAT_fw | CTTTGACACCGAGTTTTATGGATATTCCCGAAAAAGAAAGA CGG ATC CCC GGG TTA ATT AA |
HAA1_STOP483_GFP-NAT_rv | CTGTCAGTAATGTAATTGGATGATGGCGATCTTTCCGTTTA TCA GAATTCGAGCTCGTTTAAAC |
ACT1_RT_fw | CTCCACCACTGCTGAAAGAGAA |
ACT1_RT_rv | CCAAGGCGACGTAACATAGTTTT |
HAA1_RT_fw | TCGTGTGGGCGAAGTTAGC |
HAA1_RT_rv | CCAACCCCATCAATGTCAGAA |
TPO2_RT_fw | TGAGTGATCAAGAATCTGTTG |
TPO2_RT_rv | CGGTACGGTTCAATTGCTTT |
TPO3_RT_fw | TTGTGACTGGCGATCCAGAA |
TPO3_RT_rv | ACTCCAACGGATCCATGCA |
YGP1_RT_fw | TGTACAATGTTGCCCGTGTTG |
YGP1_RT_rv | GGCACCGGCGGATGA |
YRO2_RT_fw | TGGATCCCAGTCAGAGCAAAGT |
YRO2_RT_rv | ACCTGGGTGCTCCTTTTGG |
FPS1_RT_fw | ATCTGGGCCCACGTCTTG |
FPS1_RT_rv | ATGCACCCAAAGCATTTTATGA |