Metabolic engineering of indole pyruvic acid biosynthesis in Escherichia coli with tdiD

Table 2 The strains used in fine-tuning tdiD ^co expression

Strain	tdiD ^co expression plasmid	Promoter of tdiD ^co	Relative copy number of tdiD ^co expression plasmid
W3110		–	–
YL03	pTRCD	Trc	1^a
YL04	p12D	OXB12	1^a
YL05	p14D	OXB14	1^a
YL06	p16D	OXB16	1^a
YL07	p18D	OXB18	1^a
YL08	p20D	OXB20	1^a
YL09	pDRD	OXB20	2–3 [40]^b
YL10	pMOD	OXB20	6–8 [40]^b
YL11	pDMD	OXB20	Approximately 16–24 [40]^b

^aCopy number of pTRCD with the pBR322 origin and rom was identified as 1, p12D, p14D, p16D, p18D, p20D have the same copy number as pTRCD
^bpDRD, pMOD, and pDMD in YL09, YL10, and YL11, respectively had increased copy numbers

ISSN: 1475-2859