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Table 1 Strains and plasmids used in this study

From: Metabolic engineering of indole pyruvic acid biosynthesis in Escherichia coli with tdiD

 

Genotype/description

Source/references

Strain

 DH5α

lacZΔM15 endA1 recA1 relA1 gyrA96 deoR nupG λ −

Transgene Bio

 W3110

F− λ − rph-1 INV (rrnD, rrnE)

[24]

 WTRCD

W3110 with pTRCD

This study

 Sun21

W3110 Δ tyrB::FRT, ΔaspC::FRT, tyrA16::Tn10

[24]

 Zhu01

W3110 ΔtyrB, Δ aspC, ΔtnaA, tyrA16::Tn10

This study

 YL01

Sun21 with pTRCD

This study

 YL02

Sun21 with pSUFAQ

This study

 YL03

Sun21 with pTRCD and pSUFAQ

This study

 YL04

Sun21 with p12D and pSUFAQ

This study

 YL05

Sun21 with p14D and pSUFAQ

This study

 YL06

Sun21 with p16D and pSUFAQ

This study

 YL07

Sun21 with p18D and pSUFAQ

This study

 YL08

Sun21 with p20D and pSUFAQ

This study

 YL09

Sun21 with pDRD and pSUFAQ

This study

 YL10

Sun21 with pMOD and pSUFAQ

This study

 YL11

Sun21 with pDMD and pSUFAQ

This study

 YL12

Zhu01 with p20D and pSUFAQ

This study

 YL13

Sun21 with pTAD and pSUFAQ

This study

 YL14

Zhu01 with pTAD and pSUFAQ

This study

Plasmid

 pET24b-tdiD

NdeI/NotI (tdiD)

[5]

 pSUFAQ

pSU2718 derivative, aroF fbr, pheA fbr, lacI q

[24]

 pBJEI-6409

Codon-optimized genes of MEV pathway, Trc promoter

Addgene

 pUC57-D

Synthetic tdiD co

Sangon

 pUC57-18

Synthetic OXB18 promoter

Sangon

 pT7D

pET24b-tdiD derivative, T7 promoter, tdiD co

This study

 pTRCD

pT7D derivative, Trc promoter

This study

 p12D

pTRCD derivative, OXB12 promoter

This study

 p14D

pTRCD derivative, OXB14 promoter

This study

 p16D

pTRCD derivative, OXB16 promoter

This study

 p18D

pTRCD derivative, OXB18 promoter

This study

 p20D

pTRCD derivative, OXB20 promoter

This study

 pDRD

p20D derivative, Rom coding sequence deleted

This study

 pMOD

p20D derivative, origin mutation-108A(108C)

This study

 pDMD

pMOD derivative, Rom coding sequence deleted

This study

 pTAD

p20D derivative, tnaA expression cassette

This study

 pKD13

bla FRT-kan -FRT

[25]

 pKD46

bla αβ exo (red recombinase), temperature conditional replicon

[25]

 pCP20

bla cat, temperature sensitive replicon, temperature inducible FLP recombinase

[25]