From: Metabolic engineering of indole pyruvic acid biosynthesis in Escherichia coli with tdiD
 | Genotype/description | Source/references |
---|---|---|
Strain | ||
 DH5α | lacZΔM15 endA1 recA1 relA1 gyrA96 deoR nupG λ − | Transgene Bio |
 W3110 | F− λ − rph-1 INV (rrnD, rrnE) | [24] |
 WTRCD | W3110 with pTRCD | This study |
 Sun21 | W3110 Δ tyrB::FRT, ΔaspC::FRT, tyrA16::Tn10 | [24] |
 Zhu01 | W3110 ΔtyrB, Δ aspC, ΔtnaA, tyrA16::Tn10 | This study |
 YL01 | Sun21 with pTRCD | This study |
 YL02 | Sun21 with pSUFAQ | This study |
 YL03 | Sun21 with pTRCD and pSUFAQ | This study |
 YL04 | Sun21 with p12D and pSUFAQ | This study |
 YL05 | Sun21 with p14D and pSUFAQ | This study |
 YL06 | Sun21 with p16D and pSUFAQ | This study |
 YL07 | Sun21 with p18D and pSUFAQ | This study |
 YL08 | Sun21 with p20D and pSUFAQ | This study |
 YL09 | Sun21 with pDRD and pSUFAQ | This study |
 YL10 | Sun21 with pMOD and pSUFAQ | This study |
 YL11 | Sun21 with pDMD and pSUFAQ | This study |
 YL12 | Zhu01 with p20D and pSUFAQ | This study |
 YL13 | Sun21 with pTAD and pSUFAQ | This study |
 YL14 | Zhu01 with pTAD and pSUFAQ | This study |
Plasmid | ||
 pET24b-tdiD | NdeI/NotI (tdiD) | [5] |
 pSUFAQ | pSU2718 derivative, aroF fbr, pheA fbr, lacI q | [24] |
 pBJEI-6409 | Codon-optimized genes of MEV pathway, Trc promoter | Addgene |
 pUC57-D | Synthetic tdiD co | Sangon |
 pUC57-18 | Synthetic OXB18 promoter | Sangon |
 pT7D | pET24b-tdiD derivative, T7 promoter, tdiD co | This study |
 pTRCD | pT7D derivative, Trc promoter | This study |
 p12D | pTRCD derivative, OXB12 promoter | This study |
 p14D | pTRCD derivative, OXB14 promoter | This study |
 p16D | pTRCD derivative, OXB16 promoter | This study |
 p18D | pTRCD derivative, OXB18 promoter | This study |
 p20D | pTRCD derivative, OXB20 promoter | This study |
 pDRD | p20D derivative, Rom coding sequence deleted | This study |
 pMOD | p20D derivative, origin mutation-108A(108C) | This study |
 pDMD | pMOD derivative, Rom coding sequence deleted | This study |
 pTAD | p20D derivative, tnaA expression cassette | This study |
 pKD13 | bla FRT-kan -FRT | [25] |
 pKD46 | bla αβ exo (red recombinase), temperature conditional replicon | [25] |
 pCP20 | bla cat, temperature sensitive replicon, temperature inducible FLP recombinase | [25] |