Fig. 3
From: Metabolic engineering of indole pyruvic acid biosynthesis in Escherichia coli with tdiD
Metabolic engineering for IPA biosynthesis in E. coli. Genes of the catalytic enzyme are stated in the boxes. Boxes with solid lines represent E. coli inherent genes, boxes with dashed lines represent knockout genes, and boxes with bold lines represent heterogeneous genes expressed in plasmids. ANTA anthranilate, CHA chorismate, E4P erythrose-4-phosphate, DAHP 3-deoxy-d-arabino-heptulosonate-7-phosphate, HPP 4-hydroxyphenylpyruvate, IPA indole pyruvic acid, L-Phe l-phenylalanine, L-Trp l-tryptophan, L-Tyr l-tyrosine, PEP phosphoenolpyruvate, PPA phenylpyruvate. Enzymes coded by genes: aroF, DAHP synthase; aroG, DAHP synthase; aroH, DAHP synthase; aroF fbr, DAHP synthase with feedback inhibition resistance; aspC, aspartate aminotransferase; ilvE, branched chain amino acid aminotransferase; pheA, chorismate mutase/prephenate dehydratase; pheA fbr, chorismate mutase/prephenate dehydratase with feedback inhibition resistance; tdiD, l-tryptophan:phenylpyruvate aminotransferase; trpE, anthranilate synthase; tyrB, aromatic amino acid aminotransferase; tyrA, chorismate mutase/prephenate dehydrogenase