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Table 1 Codon usage of genes encoding high and low expresser variants encoding either venom peptides or the respective fusion protein

From: Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli

AA

Codon

Fc, HE, toxins

Fc, LE, toxins

Fc, HE, fusion

Fc, LE, fusion

Fc, E. coli

Arg

AGA

0

0

0

0

0.07

Arg

AGG

0

0

0.17

0.17

0.04

Arg

CGA

0

0

0

0

0.07

Arg

CGC

0.38

0.44

0.35

0.39

0.36

Arg

CGG

0

0

0

0

0.11

Arg

CGT

0.63

0.56

0.48

0.45

0.36

Asn

AAC

0.55

0.64

0.47

0.49

0.51

Asn

AAT

0.45

0.36

0.53

0.51

0.49

Cys

TGC

0.49

0.58

0.49

0.56

0.54

Cys

TGT

0.51

0.42

0.51

0.44

0.46

Glu

GAA

0.84

0.76

0.62

0.60

0.68

Glu

GAG

0.16

0.24

0.38

0.40

0.32

His

CAC

0.26

0.48

0.33

0.35

0.43

His

CAT

0.74

0.52

0.67

0.65

0.57

Ile

ATA

0

0

0

0

0.11

Ile

ATC

0.49

0.32

0.57

0.54

0.39

Ile

ATT

0.51

0.68

0.43

0.46

0.49

Phe

TTC

0.28

0.44

0.14

0.16

0.42

Phe

TTT

0.72

0.56

0.86

0.84

0.58

Ser

AGC

0.51

0.40

0.67

0.65

0.25

Ser

AGT

0.09

0.16

0.11

0.13

0.16

Ser

TCA

0.05

0.05

0.11

0.11

0.14

Ser

TCC

0.11

0.16

0.07

0.08

0.17

Ser

TCG

0.13

0.15

0.02

0.02

0.14

Ser

TCT

0.11

0.07

0.02

0.01

0.15