Fig. 6

Yields of 96 purified recombinant peptides after tag removal. Peptides are organized by increasing mass. Each original fusion tag used to express each peptide is represented by a color code (identical to Fig. 4). Yield is in milligram of oxidized peptide per litre of culture. The correct oxidation state of the purified peptide was confirmed by LC–MS and the yield of peptide per litre of culture quantified by integration of the LC peaks. a Concentration in mg/L of culture. b Yield of peptide is presented in percentage relative to the best condition to better visualize the low expressing peptides. Peptides depicted in boxes were selected for the TEV cleavage experiment (Fig. 5)