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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli

Fig. 5

TEV cleavage efficacy in various concentrations of DTT. The cleavage efficiency represents the percentage of fusion cleaved for each DTT concentration (0–2 mM) quantified by Labchip GXII (Caliper, USA) and depicted in percentages. The correct oxidation state of the purified peptide was confirmed by LC–MS (green mass corresponds to oxidized peptide, red no peptide detected). When a correct mass is detected the yield of peptide per litre of culture quantified by integration of the LC peaks is indicated in the well

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Microbial Cell Factories

ISSN: 1475-2859

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