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Table 2 Bacterial strains and plasmids utilized in this study

From: Enhanced heterologous protein productivity by genome reduction in Lactococcus lactis NZ9000

Item Genotype or phenotype Reference
Strains
 E. coli DH5α Cloning host; F-φ80lacZM15endA1 recA1 endA1 hsdR17 (rK-mK+) supE44 thi-1 gyrA 96 relA1 (lacZYA-argF)U169 deoR λ [33]
 L. monocytogenes WSLC 1019 Animal isolate (ATCC 1916), used as indicator strain [34]
 L. lactis N8 Constitutive strong promoters obtained from this strain [35]
 L. lactis NZ9000 (9 k) MG1363 pepN::nisRK, the original strain [36]
 L. lactis 9 k-1 The first DNA region L1 (about 9.7 kb) deletion in L. lactis 9 k This work
 L. lactis 9 k-2 The L2 (about 22.5 kb) deletion in L. lactis 9 k-1 This work
 L. lactis 9 k-3 The L3 (about 22.2 kb) deletion in L. lactis 9 k-2 This work
 L. lactis 9 k-4 The L4 (about 21.6 kb) deletion in L. lactis 9 k-3 This work
 L. lactis 9 k-lecC Emr, L. lactis 9 k derivative containing pLEB124-lecC This work
 L. lactis 9 k-1-lecC Emr, L. lactis 9 k-1 derivative containing pLEB124-lecC This work
 L. lactis 9 k-2-lecC Emr, L. lactis 9 k-2 derivative containing pLEB124-lecC This work
 L. lactis 9 k-3-lecC Emr, L. lactis 9 k-3 derivative containing pLEB124-lecC This work
 L. lactis 9 k-4-lecC Emr, L. lactis 9 k-4 derivative containing pLEB124-lecC This work
 L. lactis 9 k-4-P8/lecC Emr, L. lactis 9 k-4 derivative containing pLEB124-P8/lecC This work
 L. lactis 9 k-4-P5/lecC Emr, L. lactis 9 k-4 derivative containing pLEB124-P5/lecC This work
 L. lactis 9 k-rfp Cmr, L. lactis 9 k derivative containing pNZ8048-rfp This work
 L. lactis 9 k-1-rfp Cmr, L. lactis 9 k-1 derivative containing pNZ8048-rfp This work
 L. lactis 9 k-2-rfp Cmr, L. lactis 9 k-2 derivative containing pNZ8048-rfp This work
 L. lactis 9 k-3-rfp Cmr, L. lactis 9 k-3 derivative containing pNZ8048-rfp This work
 L. lactis 9 k-4-rfp Cmr, L. lactis 9 k-4 derivative containing pNZ8048-rfp This work
Plasmids
 pNZ5319 Cmr, Emr, used as knock-out vector [37]
pNZ5319L1 Cmr, Emr, the first DNA region L1 knock-out vector This work
NZ5319L2 Cmr, Emr, L2 knock-out vector This work
pNZ5319L3 Cmr, Emr, L3 knock-out vector This work
pNZ5319L4 Cmr, Emr, L4 knock-out vector This work
 pLEB124 Emr, containing strong constitutive promoter P45 [38]
 pEB690-lecC Nisinr, containing the PnisZ+Usp45-lecC+lecI fragment [23]
 pLEB124-lecC Emr, pLEB124 derivative containing the P45+PnisZ+Usp45-lecC+lecI fragment This work
 pLEB124-P8/lecC Emr, pLEB124 derivative containing the P8+PnisZ+Usp45-lecC+lecI fragment This work
 pLEB124-P5/lecC Emr, pLEB124 derivative containing the P5+PnisZ+Usp45-lecC+lecI fragment This work
 pNZ8048 Cmr [36]
 pET-28a-rfp Kanr, containing rfp gene Aviva systems biology
 pNZ8048-rfp Cmr, pNZ8048 derivative containing the rfp gene This work