Table 3 S. cerevisiae strains used in the present study

TMB4150

CEN.PK2-1C MAT a ura3-52 MAL2-8 ^C SUC2, TRP1, LEU2, HIS3, auxotrophies trp1, leu2 and his3 were reversed by transformation with PCR-amplified intact genes

Jan Knudsen, unpublished

TMB4367

TMB4150 containing pNW10, overexpressing CC-TA encoding gene, TRP1, LEU2, HIS3

[12]

TMB4369

TMB4150 containing pNW12, overexpressing CV-TA encoding gene, TRP1, LEU2, HIS3

This study

TMB4371

TMB4150 containing pNW14, overexpressing OA-TA encoding gene, TRP1, LEU2, HIS3

This study

TAM

MATa pdc1(-6,-2)::loxP pdc5(-6,-2)::loxP pdc6(-6,-2)::loxP ura3-52, selected for C₂ independence in glucose-limited chemostats and glucose-tolerant growth in batch culture

[23]

TMB4374

TAM containing pNW12, overexpressing CV-TA encoding gene, Δpdc1,5,6

This study

TMB4375

TMB4150 containing pNW12 (6 copies), overexpressing CV-TA encoding gene, TRP1, LEU2, HIS3

This study