Table 2 Plasmids used in the present study
Plasmids | Description | References |
|---|---|---|
YIpOB7 | TDH3 promoter, ADH1 terminator, XDH under PGK1 promoter, with PGK1 terminator, URA3 gene, AMP resistance gene | [54] |
YIpNW | YIpOB7 without XDH, TDH3 promoter, ADH1 terminator, PGK1 promoter, PGK1 terminator, URA3 gene, AMP resistance gene | This study |
pNW10 | CC-TA ORF cloned in YIpNW between TDH3 promoter and ADH1 terminator, URA3 gene, AMP resistance gene | [12] |
pUC57-CV | Codon-optimized CV-TA gene | GenScript, NJ, USA |
pUC57-OA | Codon-optimized OA-TA gene | GenScript, NJ, USA |
pNW12 | CV-TA ORF between TDH3 promoter and ADH1 terminator, URA3 gene, AMP resistance gene | This study |
pNW14 | OA-TA ORF between TDH3 promoter and ADH1 terminator, URA3 gene, AMP resistance gene | This study |