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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Improvement of whole-cell transamination with Saccharomyces cerevisiae using metabolic engineering and cell pre-adaptation

Fig. 1

a Whole-cell kinetic resolution of racemic 1-PEA with (S)-selective ω-transaminase to (R)-1-PEA and acetophenone. The mechanism for enzymatic transamination consists of two half-reactions under which the co-factor PLP is regenerated, and the amine acceptor/donor is converted into the corresponding amine/ketone. PLP and the amine acceptor pyruvate is formed in the cell by concomitant conversion of glucose. b Whole-cell asymmetric conversion of acetophenone to (S)-1-PEA by (S)-selective ω-transaminase. The co-product pyruvate is removed from the system by further conversion catalysed by PDC and ADH resulting in formation of CO2 and ethanol

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