Table 3 Primers
Name | Sequence (5′→3′) | Restriction enzyme | Length (bp) |
|---|---|---|---|
Ace2-P8 | 5′-TATTCTAGAATGGACCTCCGGCAAGCATGT-3′ | XbaI | 30 |
Ace2-P6 | 5′-GCAAAGCTTTCGTCTGTTTTTGATGACTTC-3′ | HindIII | 30 |
hph-P1 | 5′-GCGAAGCTTGAGAGCTACCTTACATCAAT-3′ | HindIII | 29 |
hph-P2 | 5′-GTGAAGCTTATACCCCAGTCCAGATCATG-3′ | HindIII | 29 |
pki1-P1 | 5′-GACGAAGACCTGACTCGTGA-3′ | XbaI, EcoRI | 20 |