Engineering Aspergillus niger for galactaric acid production: elimination of galactaric acid catabolism by using RNA sequencing and CRISPR/Cas9

Table 1 Genes selected for deletion based on RNA sequencing of A. niger wild type strain cultivated on galactaric acid

Protein ID	Galactaric acid			InterPro/KOG prediction
Protein ID	0 h	5 h	18 h	InterPro/KOG prediction
39114	1	5497	4219	AMP-dependent synthetase and ligase, α-aminoadipate-semialdehyde dehydrogenase
1090836	1	5239	6627	Aldo/keto reductase
1117792	42	3239	1050	Alcohol dehydrogenase, zinc-binding
1141260	132	805	747	Short-chain dehydrogenase/reductase
1121140	51	545	1162	FAD-dependent oxidoreductase
1146483	2	641	1661	Mandelate racemase/muconate lactonizing enzyme
1170646	1	260	164	d-isomer specific 2-hydroxyacid dehydrogenase

Transcript levels are presented as fragments per kilobase of exon per million fragments mapped (FPKM). The protein ID numbers refer the numbers from the Join Genome Institute, MycoCosm, A. niger ATCC 1015 v.4.0 database

ISSN: 1475-2859