Skip to main content

Table 1 Primers, plasmids, and Escherichia coli strains used in this study

From: Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts

Names

Descriptions

References

Primersa

 

This study

 PaispS-F

AGACGGTCTGCCAATTATGAACC

This study

 PaispS-R

CTCTAGATTATCTCTCAAAGGGTAGAATAG

This study

 PtispS-F

GCC ATG GCATGTTCTGTAAGCACAG

This study

 PtispS-R

CTCTAGATTATCTCTCAAAGGGTAGAATAG

This study

 KispS-F

TCTCTGGAAAATGACCTTAAGG

This study

 KispS-R

CTCTAGATTAGCAGCCGGATCCCACGTAC

This study

 sPtispS-F

GCC ATG GCTTGCTCTGTATCCAC

This study

 sPtispS-R

CTCTAGATTAGCGTTCGAACGGCAGAATTG

This study

 MVA-F

GTCTAGATACCTGACGCTTTTTATCGCAAC

This study

 MVA-R

GTCTAGAGTTTCGATAAGAGCGAACGG

This study

Plasmids

 pTrc99A

Ptrc expression vector, pBR322 origin, lacIq, Ampr

[23]

 pTrc99S

pTrc99A containing strong RBS

This study

 pSTV28

Plac expression vector, pACYC184 origin, lacZ, Cmr

Takara

 pT-PaispS

pTrc99A containing ispS from P. alba

This study

 pT-PtispS

pTrc99A containing ispS from P. trichocarpa

This study

 pT-KispS

pTrc99A containing ispS from Kudzu vine

This study

 pT-sPtispS

pTrc99A containing codon optimized ispS from P. trichocarpa

This study

 pTS-sPtispS

pTrc99S containing codon optimized ispS from P. trichocarpa

This study

 pTS-sPt-MVA

pTS-sPtispS containing MVA pathway from plasmid pS-NA

This study

 pS-NA

pSTV28 containing mvaE and mvaS from E. faecalis; mvaK1, mvaK2, and mvaD from S. pneumoniae; and idi from E. Coli

[19]

Strains

 MG1655

E. coli K-12; Fλ, ilvG, rfb-50, rph-1

ATCC 700926

 AceCo

MG1655 ΔackA-pta, poxB, ldhA, dld, adhE, pps, atoDA

Additional file 1: Table S1

 DH5α

E. coli K-12; F, φ80dlacZΔM15, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17 (rK mK+), phoA, supE44, λ, thi-1

ATCC 98040

 MGpPapM

MG1655 harboring pT-PaispS and pS-NA

This study

 MGpKpM

MG1655 harboring pT-KispS and pS-NA

This study

 MGpPtpM

MG1655 harboring pT-PtispS and pS-NA

This study

 MGpsPtpM

MG1655 harboring pTS-sPtispS and pS-NA

This study

 MGpsPtM

MG1655 harboring pTS-sPtispS-MVA

This study

 ApsPtM

AceCo harboring pTS-sPtispS-MVA

This study

  1. aStart codons are presented in bold, and restriction sites are underlined