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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts

Fig. 5

a Pathway of byproduct formation from acetyl-CoA and pyruvate in Escherichia coli. Strikeouts indicate deleted genes in the AceCo strain. The deleted genes and their corresponding enzymes are as follows: ldhA lactate dehydrogenase; dld d-lactate dehydrogenase; pps phosphoenolpyruvate synthetase; poxB pyruvate oxidase; adhE aldehyde-alcohol dehydrogenase; pta phosphate acetyltransferase; ackA acetate kinase; atoD acetoacetyl-CoA transferase; atoA acetoacetyl-CoA transferase. b Analysis of extracellular metabolites (acetate, lactate, pyruvate, and mevalonate) accumulated during the culture of strain MGpsPtM (wild-type MG1655 harboring pTS-sPtispS-MVA) shown in Fig. 4 (open squares) and the strain ApsPtM (the knockout mutant AceCo harboring the same plasmid) shown in Fig. 6 (closed squares) after induction with 0.01 mM IPTG

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