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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts

Fig. 1

Isoprene synthesis pathway (a) and gene clusters used in this study (b). The light and dark gray arrows represent the wild-type isoprene synthase (PtispS) from Populus trichocarpa and its codon-optimized ispS (sPtispS) for expression in Escherichia coli, respectively. The white and dark gray ovals indicate the original RBS of the pTrc99A vector and the modified RBS with higher ribosomal affinity, respectively. The white arrows represent the genes of the MVA pathway (mvaK1, mvaD, and mvaK2 from Streptococcus pneumoniae; mvaE and mvaS from Enterococcus faecalis; and idi from Escherichia coli)

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