Fig. 5
From: Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR
Plasmid copy number (PCN) of selected sub-populations. Two E. coli strains with a different degree of heterogeneity regarding EGFP intensity were used for further characterization of PCN. a For E. coli DH5α λpir p244-AEB, three sub-populations of low (−), intermediate (+) and high EGFP fluorescence (++) were chosen. For E. coli DH5α λpir p254-AEB, two sub-populations were chosen according to a low (−) and a high EGFP intensity peak (+). For ddPCR, 1000 cells of each sub-population were sorted. b PCN of sorted sub-populations as described before. R1, R2—independent biological replicates. Bars represent mean and standard deviation