Fig. 2
From: Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR
EGFP intensity of seven plasmid-carrying E. coli strains. The p2X4-AEB vector series was analyzed via flow cytometry to evaluate productivity and population heterogeneity in E. coli DH5α λpir. Representative samples were taken from a batch cultivation for 24 h with induction by IPTG after 2 h. The first row (-AEB) shows no-fluorescence control strains carrying the p2X4 plasmid series devoid of styA-EGFP styB. a.u.—arbitrary units, dashed line—threshold used for discrimination of EGFP negative and positive cells