Fig. 6
From: Microbial production of next-generation stevia sweeteners
LC-MS analysis of the steviol glucosides present in the S. cerevisiae clones expressing UGT76G1 variants. a, b UGT76G1 variants found to increase Reb D accumulation (High Reb D variants); c, d UGT76G1 variants examined for increased Reb M accumulation (High Reb M variants). The number of clones identified to harbour the same UGT76G1 mutation is shown. For the three variants Thr146Pro, Leu257Arg and Thr55Lys, no error bars are shown because only a single sample of each was screened due to background strain variation during transformation. Two sets of wild-type controls (WT) were analysed in triplicate with each test. Steviol, steviol-19-O-glucoside (19-SMG) and 1,3-bioside were not detected in any of the samples. The steviol glucosides in a and c were quantified based on authentic reference compounds whereas those in b and d were assessed by their respective AUC units (area under curve)