Fig. 2

1.3-Glucosylation reactions catalyzed by wild-type UGT76G1. Substrates tested were: a rubusoside, b 1,2-bioside, c 1,2-stevioside, d Reb A, e Reb D and f Reb E. Each substrate was administered at an initial concentration of 0.1 mM. Substrate conversion and formation of 1,3-mono-, di- and tri-glucosylated products are shown in blue, red and green traces, respectively. Reb I resulting from glucosylation of Reb A is not depicted due to partial co-elution with Reb A and 1,2-stevioside which prevents integration of the Reb I peak in the LC-MS results. The AUC units depicted do not offer the ability to make quantitative comparisons between different components