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Table 1 Measured OTRmax and maximum oxygen transfer capacities calculated according to an empirical correlation (OTRmax,Lattermann) as well as calculated kLa values during E. coli cultivations on synthetic Wilms-MOPS medium in 48 deep-well MTPs

From: Quasi-continuous parallel online scattered light, fluorescence and dissolved oxygen tension measurement combined with monitoring of the oxygen transfer rate in each well of a shaken microtiter plate

Filling volume (µL) Number of cultivations (−) OTRmax (mmol L−1 h−1) OTRmax,Lattermann (mmol L−1 h−1) kLa (h−1)
700 5 54 ± 0.8 49 ± 7.3 329 ± 20
800 5 48 ± 1.4 45 ± 6.7 291 ± 18
900 6 43 ± 0.7 42 ± 6.3 264 ± 16
1000 5 39 ± 1.1 39 ± 5.9 245 ± 15
1100 5 38 ± 0.8 37 ± 5.5 229 ± 14
1200 4 36 ± 1.1 35 ± 5.2 216 ± 13
1300 5 34 ± 0.5 33 ± 5.0 203 ± 13
1400 6 32 ± 1.3 32 ± 4.7 198 ± 12