Quasi-continuous parallel online scattered light, fluorescence and dissolved oxygen tension measurement combined with monitoring of the oxygen transfer rate in each well of a shaken microtiter plate

Table 1 Measured OTR_max and maximum oxygen transfer capacities calculated according to an empirical correlation (OTR_{max,Lattermann}) as well as calculated k_La values during E. coli cultivations on synthetic Wilms-MOPS medium in 48 deep-well MTPs

Filling volume (µL)	Number of cultivations (−)	OTR_max (mmol L⁻¹ h⁻¹)	OTR_{max,Lattermann} (mmol L⁻¹ h⁻¹)	k_La (h⁻¹)
700	5	54 ± 0.8	49 ± 7.3	329 ± 20
800	5	48 ± 1.4	45 ± 6.7	291 ± 18
900	6	43 ± 0.7	42 ± 6.3	264 ± 16
1000	5	39 ± 1.1	39 ± 5.9	245 ± 15
1100	5	38 ± 0.8	37 ± 5.5	229 ± 14
1200	4	36 ± 1.1	35 ± 5.2	216 ± 13
1300	5	34 ± 0.5	33 ± 5.0	203 ± 13
1400	6	32 ± 1.3	32 ± 4.7	198 ± 12

ISSN: 1475-2859