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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Quasi-continuous parallel online scattered light, fluorescence and dissolved oxygen tension measurement combined with monitoring of the oxygen transfer rate in each well of a shaken microtiter plate

Fig. 6

Measurement setup and operating principle. a Each well of the MTP is monitored via fluorescence, scattered light and dissolved oxygen tension (DOT) through the transparent bottom. By means of the µRAMOS device the oxygen concentration in the headspace of each well is monitored and the oxygen transfer rate (OTR) is determined. b The measurement phases of the µRAMOS and the BioLector device must be coordinated to avoid interferences between the optical measurements. Fluorescence and DOT measurements can be obtained simultaneously with measurements of the headspace oxygen concentration (for OTR evaluation) without interference. Scattered light and oxygen measurements in the gas phase (for OTR evaluation), can only be performed in alternating sequence

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